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Int J Mol Epidemiol Genet 2011;2(1):73-77.
Original Article
Molecular typing of Salmonella spp isolated from food handlers and animals in
Nigeria
Stella I. Smith, Muinah A. Fowora, Helen A. Goodluck, Francisca O. Nwaokorie, Olusimbo A. Aboaba, Bolanle Opere
Molecular Biology and Biotechnology Division, Nigerian Institute of Medical Research, 6 Edmond Crescent, PMB 2013, Yaba,
Lagos, Nigeria; Department of Microbiology, University of Lagos, Nigeria; Department of Microbiology, Lagos State University,
PMB 1087, Ojo, Lagos, Nigeria.
Received September 8, 2010; accepted December 18, 2010; Epub December 28, 2010; published January 1, 2011
Abstract: A total of 61 isolates of Salmonella spp (made up of 26 clinical isolates and 35 food handler and animal isolates)
were typed by RAPD-PCR for the purpose of screening for epidemiologically related isolates. The RAPD -PCR typing method
used comprised six primers namely 787, 797, 784, 1254, RAPD 1 and RAPD 2 but 784 and 1254 did not produce
discriminatory patterns and so were dropped. From the 61 strains, RAPD fingerprinting with primers RAPD 1, 2 produced 22
and 24 fingerprint patterns respectively. RAPD fingerprinting with primers 787, 797 produced 17, 11 fingerprinting patterns
respectively. Combinations of the two RAPD 1 and 2 primers increased the discrimination of Salmonella strains to 32 patterns
rather than the other primers used. Primer 797 was the least discriminatory. This study showed that the RAPD 1 and 2 primers
would be useful for epidemiological typing of the Salmonella spp in Nigeria. (IJMEG1009002).
Key words: Salmonella, food handlers, animals, RAPD-PCR
Full Text PDF
Address all correspondence to:
Stella I. Smith, PhD
Molecular Biology and Biotechnology Division
Nigerian Institute of Medical Research
6 Edmond Crescent, PMB 2013, Yaba
Lagos, Nigeria
Telephone: +2341 4822269
Fax: +234 1 3425171
E-mail: stellaismith@yahoo.com
Original Article
Molecular typing of Salmonella spp isolated from food handlers and animals in
Nigeria
Stella I. Smith, Muinah A. Fowora, Helen A. Goodluck, Francisca O. Nwaokorie, Olusimbo A. Aboaba, Bolanle Opere
Molecular Biology and Biotechnology Division, Nigerian Institute of Medical Research, 6 Edmond Crescent, PMB 2013, Yaba,
Lagos, Nigeria; Department of Microbiology, University of Lagos, Nigeria; Department of Microbiology, Lagos State University,
PMB 1087, Ojo, Lagos, Nigeria.
Received September 8, 2010; accepted December 18, 2010; Epub December 28, 2010; published January 1, 2011
Abstract: A total of 61 isolates of Salmonella spp (made up of 26 clinical isolates and 35 food handler and animal isolates)
were typed by RAPD-PCR for the purpose of screening for epidemiologically related isolates. The RAPD -PCR typing method
used comprised six primers namely 787, 797, 784, 1254, RAPD 1 and RAPD 2 but 784 and 1254 did not produce
discriminatory patterns and so were dropped. From the 61 strains, RAPD fingerprinting with primers RAPD 1, 2 produced 22
and 24 fingerprint patterns respectively. RAPD fingerprinting with primers 787, 797 produced 17, 11 fingerprinting patterns
respectively. Combinations of the two RAPD 1 and 2 primers increased the discrimination of Salmonella strains to 32 patterns
rather than the other primers used. Primer 797 was the least discriminatory. This study showed that the RAPD 1 and 2 primers
would be useful for epidemiological typing of the Salmonella spp in Nigeria. (IJMEG1009002).
Key words: Salmonella, food handlers, animals, RAPD-PCR
Full Text PDF
Address all correspondence to:
Stella I. Smith, PhD
Molecular Biology and Biotechnology Division
Nigerian Institute of Medical Research
6 Edmond Crescent, PMB 2013, Yaba
Lagos, Nigeria
Telephone: +2341 4822269
Fax: +234 1 3425171
E-mail: stellaismith@yahoo.com
