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Int J Mol Epidemiol Genet 2011;2(1):56-60.
Original Article
Absence of the pathogenicity island-like DNA segment associated with
Chinese outbreak strain of Streptococcus suis serotype 2 in the United States
isolates.
Sarah Schmid, Michael O’Connor, Ogi Okwumabua
Department of Pathobiological Sciences, School of Veterinary Medicine, Wisconsin Veterinary Diagnostic Laboratory, and
College of Agriculture and Life Sciences, University of Wisconsin, Madison, USA.
Received November 1, 2010; accepted December 22, 2010; Epub December 26, 2010; published January 1, 2011
Abstract: To determine if the 2005 Chinese outbreak strain of Streptococcus suis is circulating in the United States, three
different PCR primer-pairs derived from the nucleotide sequences surrounding and internal to the unique PAI-like DNA
segment of the Chinese outbreak strain (strain 05ZYH33) were used to screen 290 S. suis isolates obtained from different
locations. The first primer pair amplified an approximately 1000-bp fragment from 47 (16%) of the United States isolates and
the second amplified an 1800-bp fragment from 23 (8%) of the isolates. Nucleotide sequences of the amplicons shared
identity with those of strain 05ZYH33. The third primer pair amplified a 716-bp amplicon from the DNA of strain 05ZYH33 only.
These observations demonstrated that the PAI homologue of strain 05ZYH33 is absent in the United States isolates tested and
suggested that the PCR method may be useful for active surveillance to monitor possible spread of the highly invasive strain.
(IJMEG1011001).
Key words: Streptococcus suis, Pathogenicity Island, Toxic Shock, Polymerase Chain Reaction, Surveillance
Full Text PDF
Address all correspondence to:
Ogi Okwumabua, PhD
Department of Pathobiological Sciences
School of Veterinary Medicine,
University of Wisconsin
Madison, USA.
Tel: (608) 262-4168; Fax: (847) 574-8085
E-mail address: ogi.okwumabua@wvdl.wisc.edu
Original Article
Absence of the pathogenicity island-like DNA segment associated with
Chinese outbreak strain of Streptococcus suis serotype 2 in the United States
isolates.
Sarah Schmid, Michael O’Connor, Ogi Okwumabua
Department of Pathobiological Sciences, School of Veterinary Medicine, Wisconsin Veterinary Diagnostic Laboratory, and
College of Agriculture and Life Sciences, University of Wisconsin, Madison, USA.
Received November 1, 2010; accepted December 22, 2010; Epub December 26, 2010; published January 1, 2011
Abstract: To determine if the 2005 Chinese outbreak strain of Streptococcus suis is circulating in the United States, three
different PCR primer-pairs derived from the nucleotide sequences surrounding and internal to the unique PAI-like DNA
segment of the Chinese outbreak strain (strain 05ZYH33) were used to screen 290 S. suis isolates obtained from different
locations. The first primer pair amplified an approximately 1000-bp fragment from 47 (16%) of the United States isolates and
the second amplified an 1800-bp fragment from 23 (8%) of the isolates. Nucleotide sequences of the amplicons shared
identity with those of strain 05ZYH33. The third primer pair amplified a 716-bp amplicon from the DNA of strain 05ZYH33 only.
These observations demonstrated that the PAI homologue of strain 05ZYH33 is absent in the United States isolates tested and
suggested that the PCR method may be useful for active surveillance to monitor possible spread of the highly invasive strain.
(IJMEG1011001).
Key words: Streptococcus suis, Pathogenicity Island, Toxic Shock, Polymerase Chain Reaction, Surveillance
Full Text PDF
Address all correspondence to:
Ogi Okwumabua, PhD
Department of Pathobiological Sciences
School of Veterinary Medicine,
University of Wisconsin
Madison, USA.
Tel: (608) 262-4168; Fax: (847) 574-8085
E-mail address: ogi.okwumabua@wvdl.wisc.edu
