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Int J Mol Epidemiol Genet 2011;2(1):78-94
Original Article
Maternal peripheral blood gene expression in early pregnancy and
preeclampsia
Daniel A. Enquobahrie, Chunfang Qiu, Seid Y. Muhie, Michelle A. Williams
Center for Perinatal Studies, Swedish Medical Center, Seattle, WA, USA; Cardiovascular Health Research Unit, University of
Washington, Seattle, WA, USA; Department of Epidemiology, University of Washington, Seattle, WA, USA; Walter Reed Army
Institute of Research, Silver Spring, MD, USA.
Received November 3, 2010; accepted December 28, 2010; Epub December 29, 2010; published January 1, 2011
Abstract: We investigated associations of early pregnancy maternal peripheral blood gene expression with preeclampsia. In a
nested case control study, gene expression of peripheral blood, collected at 16weeks of gestation on average from 16 women
destined to develop preeclampsia and 16 women who had normotensive pregnancies was profiled using Affymetrix GeneChip
Arrays. Fold change and Student’s T-test analyses were used to compare differential gene expression across the groups.
Functions and functional relationships as well as common regulatory sequences of differentially expressed genes were
investigated. Genes participating in abnormal placentation (e.g COL1A1), immune/inflammation response (e.g. IKBKB) and
cellular development (including cell cycle) (e.g. RB1) were differentially expressed in early pregnancy peripheral blood in
preeclampsia. We identified transcription factors (i.e. Sp1, MAZ and MZF1) that may account for co-expression of differentially
expressed genes. Preeclampsia is associated with differential gene expression in early pregnancy peripheral blood.
(IJMEG1011002).
Keywords: Preeclampsia, early pregnancy, gene, expression
Full Text PDF
Address all correspondence to:
Daniel A. Enquobahrie, MD, PhD
Center for Perinatal Studies
Swedish Medical Center
1124 Columbia Street, Suite 750
Seattle, WA 98104
Tel: 206 215 2595; Fax: 206 215 6995
E-mail: danenq@u.washington.edu
Original Article
Maternal peripheral blood gene expression in early pregnancy and
preeclampsia
Daniel A. Enquobahrie, Chunfang Qiu, Seid Y. Muhie, Michelle A. Williams
Center for Perinatal Studies, Swedish Medical Center, Seattle, WA, USA; Cardiovascular Health Research Unit, University of
Washington, Seattle, WA, USA; Department of Epidemiology, University of Washington, Seattle, WA, USA; Walter Reed Army
Institute of Research, Silver Spring, MD, USA.
Received November 3, 2010; accepted December 28, 2010; Epub December 29, 2010; published January 1, 2011
Abstract: We investigated associations of early pregnancy maternal peripheral blood gene expression with preeclampsia. In a
nested case control study, gene expression of peripheral blood, collected at 16weeks of gestation on average from 16 women
destined to develop preeclampsia and 16 women who had normotensive pregnancies was profiled using Affymetrix GeneChip
Arrays. Fold change and Student’s T-test analyses were used to compare differential gene expression across the groups.
Functions and functional relationships as well as common regulatory sequences of differentially expressed genes were
investigated. Genes participating in abnormal placentation (e.g COL1A1), immune/inflammation response (e.g. IKBKB) and
cellular development (including cell cycle) (e.g. RB1) were differentially expressed in early pregnancy peripheral blood in
preeclampsia. We identified transcription factors (i.e. Sp1, MAZ and MZF1) that may account for co-expression of differentially
expressed genes. Preeclampsia is associated with differential gene expression in early pregnancy peripheral blood.
(IJMEG1011002).
Keywords: Preeclampsia, early pregnancy, gene, expression
Full Text PDF
Address all correspondence to:
Daniel A. Enquobahrie, MD, PhD
Center for Perinatal Studies
Swedish Medical Center
1124 Columbia Street, Suite 750
Seattle, WA 98104
Tel: 206 215 2595; Fax: 206 215 6995
E-mail: danenq@u.washington.edu
