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Int J Mol Epidemiol Genet 2013;4(1):70-76
Original Article
Superantigen involvement and susceptibility factors in Kawasaki dis-ease:
profiles of TCR Vβ2+ T cells and HLA-DRB1, TNF-α and ITPKC genes among
Filipino patients
Magdalena F Natividad, Celia Aurora T Torres-Villanueva, Cynthia P Saloma
National Institute of Molecular Biology and Biotechnology, University of the Philippines, Diliman, Quezon City; Department of
Microbiology and Parasitology, Far Eastern University-Dr. Nicanor Reyes Medical Foundation, Quezon City, Philippines; Current
affiliation: CSIRO Marine and Atmospheric Research Division, Melbourne, Australia
Received February 19, 2013; Accepted March 6, 2013; Epub March 18, 2013; Published March 28, 2013
Abstract: Superantigens and genetic factors may play roles in the etiology and susceptibility to Kawasaki disease (KD). To
investigate these roles, percentages of TCR-Vβ2+ T cells were compared by flow cytometry using anti-Vβ2 monoclonal
antibodies and genotyping was done on HLA-DRB1 exon 2, the -308 site of the TNF-α promoter region, and ITPKC SNP
rs28493229 by polymerase chain reaction followed by direct sequencing. There were higher percentages of Vβ2+ T-cells in KD
patients (9.5 ± 2.15%) compared to healthy controls (7.25 ± 1.48%) (P<0.05, Student’s t-test, n=6-8/group). However, no
polymorphisms were observed in exon 2 of HLA-DRB1 and in the -308 region of the TNF-α promoter. The ITPKC SNP
rs28493229 G/C polymorphism was observed in 1 KD patient and 4 healthy controls. This study suggests that KD etiology may
be associated with a superantigen and that HLA-DRB1 exon2, TNF-α -308 region and ITPKC SNP rs28493229 may not be
associated with KD. This is the first study investigating Vβ2+ T cells and candidate genes involvement among Filipino KD
patients. (IJMEG1302004).
Keywords: Superantigens, HLA-DRB1, TNF-α, ITPKC, Kawasaki disease
Address correspondence to: Magdalena F Natividad, Department of Microbiology and Parasitology, Far Eastern University-Dr.
Nicanor Reyes Medical Foundation, Fairview, Quezon City, Philippines. Fax: 63 (02) 9835906; E-mail: mfnat118@yahoo.com
Original Article
Superantigen involvement and susceptibility factors in Kawasaki dis-ease:
profiles of TCR Vβ2+ T cells and HLA-DRB1, TNF-α and ITPKC genes among
Filipino patients
Magdalena F Natividad, Celia Aurora T Torres-Villanueva, Cynthia P Saloma
National Institute of Molecular Biology and Biotechnology, University of the Philippines, Diliman, Quezon City; Department of
Microbiology and Parasitology, Far Eastern University-Dr. Nicanor Reyes Medical Foundation, Quezon City, Philippines; Current
affiliation: CSIRO Marine and Atmospheric Research Division, Melbourne, Australia
Received February 19, 2013; Accepted March 6, 2013; Epub March 18, 2013; Published March 28, 2013
Abstract: Superantigens and genetic factors may play roles in the etiology and susceptibility to Kawasaki disease (KD). To
investigate these roles, percentages of TCR-Vβ2+ T cells were compared by flow cytometry using anti-Vβ2 monoclonal
antibodies and genotyping was done on HLA-DRB1 exon 2, the -308 site of the TNF-α promoter region, and ITPKC SNP
rs28493229 by polymerase chain reaction followed by direct sequencing. There were higher percentages of Vβ2+ T-cells in KD
patients (9.5 ± 2.15%) compared to healthy controls (7.25 ± 1.48%) (P<0.05, Student’s t-test, n=6-8/group). However, no
polymorphisms were observed in exon 2 of HLA-DRB1 and in the -308 region of the TNF-α promoter. The ITPKC SNP
rs28493229 G/C polymorphism was observed in 1 KD patient and 4 healthy controls. This study suggests that KD etiology may
be associated with a superantigen and that HLA-DRB1 exon2, TNF-α -308 region and ITPKC SNP rs28493229 may not be
associated with KD. This is the first study investigating Vβ2+ T cells and candidate genes involvement among Filipino KD
patients. (IJMEG1302004).
Keywords: Superantigens, HLA-DRB1, TNF-α, ITPKC, Kawasaki disease
Address correspondence to: Magdalena F Natividad, Department of Microbiology and Parasitology, Far Eastern University-Dr.
Nicanor Reyes Medical Foundation, Fairview, Quezon City, Philippines. Fax: 63 (02) 9835906; E-mail: mfnat118@yahoo.com
